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Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through <t>the</t> <t>JC‐10</t> assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.
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Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through <t>the</t> <t>JC‐10</t> assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.
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Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through <t>the</t> <t>JC‐10</t> assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.
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Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through <t>the</t> <t>JC‐10</t> assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.
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Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through <t>the</t> <t>JC‐10</t> assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.
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Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through <t>the</t> <t>JC‐10</t> assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.
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Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through the JC‐10 assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through the JC‐10 assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.

Article Snippet: After treating the cells with PCG, the plates were incubated again for 48 h. Subsequently, the cells were treated with 5 μM JC‐10 (AdipoGen Life Sciences, San Diego, USA) in DMSO and incubated for 30 min [ ].

Techniques: Membrane, Cell Culture, Control